Biotinylated Cynomolgus MAdCAM-1 Protein, His, Avitag™ (MALS verified)

The mucosal addressin cell adhesion molecule-1 (MAdCAM-1) takes a key role in the endothelial adhesion and migration of lymphocytes to sites of inflammation in inflammatory disease. In vitro and in vivo data indicate that blockade of the adhesion molecule mucosal address in cell adhesion molecule (MAdCAM) pathway decreases leukocyte homing to the gut by inhibiting the interaction between MAdCAM and its ligand the α4β7 integrin expressed on lymphocytes.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 32.2 kDa. The protein migrates as 60-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

XP_045235080.1

Expression Region

Gln 22 - Lys 291

Host

HEK293

Target

MAdCAM-1

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Cynomolgus

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT7-H52W4) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus MAdCAM-1 Protein, His,Avitag (Cat. No. MAM-C82E3) with a linear range of 1-39 ng/mL (QC tested).