JC-1

JC-1 is a fluorescent lipophilic carbocyanine dye. It has been used to measure mitochondrial membrane potential. JC 1 can be used in a probe for measuring mitochondrial membrane potential by flow cytometry. JC-1 fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers. JC-1 (2.5 μM) exposed to murine L1210 lymphoblasts, can be detected the presence of both cytoplasmic JC-1 monomer and mitochondrial J-aggregates in these cells.