Recombinant Rat Mucosae-associated Epithelial Chemokine/CCL28
Product Specifications
Background
CCL28 belongs to CC family chemokine, also named mucosae-associated epithelial chemokine (MEC) . The CCL28 mRNA is highly expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. The protein signals through CCR3 and CCR10, and chemotactic for resting CD4, CD8 T-cells and eosinophils. CCL28 shares the most homology with CCL27/CTACK.
Product Name Alternative
CCK1
Target
SEAILPIASS CCTEVSHHIP RRLLERVNSC SIQRADGDCD LAAVILHVKR RRICVSPHNP TLKRWMSASE MKNGKENLCP RKKQDSGKDR KGHTPRKHGK HGTRRIHGTH DHEAPR
Purification
> 96 % by SDS-PAGE and HPLC analyses.
Endotoxin
Less than 1 EU/ug of rRtMEC/CCL28 as determined by LAL method.
Bioactivity
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 5.0-50 ng/ml.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculated Molecular Weight
Approximately 13.1 kDa, a single non-glycosylated polypeptide chain containing 116 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 200 mM NaCl.
Host or Source
Escherichia coli
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