Anti-PfHsp70 Antibody

Rabbit Polyclonal Antibody specific to PfHsp70

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

HSP70, 74.3 kDa protein, Cytoplasmic antigen

UniProt

P11144

Accession Number

M19753

Host

Rabbit

Reactivity

P. Facilparum

Immunogen

His-tagged C-terminal amino acids 365-681 of P. falciparum Hsp70 (Accession no. M19753).

Target Antigen

Heat shock 70 kDa protein

Target

PfHsp70

Clonality

Polyclonal

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Heat Shock& Stress Proteins

Purification Method

Purified by Protein A affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at 0.1-1ug/mL. A band of ~70kDa is detected. <br><br>These are recommended concentrations. <br><br>Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes P. falciparum Hsp70.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide. Purified by Protein A affinity chromatography.

Buffer pH

pH 7.4

Target Background

Plasmodium falciparum expresses two prominent heat shock proteins: heat shock protein 70 (PfHsp70) and heat shock protein 90 (PfHsp90). Both of these proteins have been implicated in the development and pathogenesis of malaria. PfHsp70-1 is the major cytosolic Hsp70 in Plasmodium falciparum. It is abundantly expressed in the blood stages of the parasite and appears to constitute approx. 1-2% of total parasite protein. It is found in P. falciparum in complexes with PfHsp90 and PfHsp40. The transition from the cold blooded mosquito vector to a host represents physiological stress to the parasite, and, additionally, malaria blood stage infection is characterized by intense fever periods. In recent years, it has become clear that heat shock proteins play an essential role during the parasite's life cycle.