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Canine Leukemia Inhibitory Factor (LIF) ELISA

The Canine LIF ELISA contains capture antibody, standard, and detection antibody for development of a Canine LIF ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. This allows for complete flexibility to provide optimal dynamic range and sensitivity based on your sample type. The Canine LIF-A ELISA can also be used to measure Dingo and Red Fox LIF. Alternate names include LIF, CDF, DIA, HILDA, MLPLI, leukemia inhibitory factor, interleukin 6 family cytokine, LIF interleukin 6 family cytokine. The quantities of components provided are not matched. Components may also be purchased separately. For research use only.

Product Specifications

Background

Leukemia inhibitory factor, or LIF, is a member of the IL-6 family. LIF affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. Leukemia inhibitory factor derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to LIF include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. p53 regulated LIF has been shown to facilitate implantation in the mouse model and possibly in humans. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. LIF is typically added to stem cell culture medium to reduce spontaneous differentiation.

Homology

Canis lupus dingo (dingo), Canis lupus familiaris (dog), Vulpes lagopus (Arctic fox), Vulpes vulpes (red fox)

Storage Temperature

2°C to 8°C or -20°C

Scientific Category

ELISA

Available Sizes

Frequently Asked Questions

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