Bovine IL-1 Receptor Antagonist (IL-1RA) ELISA

The Bovine IL-1 Receptor Antagonist (IL-1RA) ELISA contains capture antibody, protein standard, and detection antibody for development of a Bovine IL-1RA ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. This allows for complete flexibility to provide optimal dynamic range and sensitivity based on your sample type. The Bovine IL-1RA ELISA can also be used to measure American Buffalo, Water Buffalo, Yak, and Zebu IL-1RA. The quantities of components provided are not matched. Components may also be purchased separately. For research use only.

Product Specifications

Background

IL1RA is secreted by various types of cells including immune cells, epithelial cells, and adipocytes, and is a natural inhibitor of the pro-inflammatory effect of IL-1β. It inhibits the activities of IL-1 alpha and IL-1 beta, and modulates a variety of interleukin 1 related immune and inflammatory responses. The IL-1 family of cytokines encompasses eleven proteins that each share a similar β-barrel structure and bind to Ig-like receptors. Several of the well characterized members of the IL-1-like cytokines play key roles in the development and regulation of inflammation. IL-1α, IL-1β, and IL-18 (IL-1F4) are well-known inflammatory cytokines active in the initiation of the inflammatory reaction and in driving Th1 and Th17 inflammatory responses. In contrast, IL-1 receptor antagonist (IL-1ra; IL-1F3) and IL-36 receptor antagonist (IL-36ra; IL-1F5) reduce inflammation by blocking the binding of the agonist receptor ligands. IL-33 (IL-1F11) is thought to function as an 'alarmin' released following cell necrosis to alerting the immune system to tissue damage or stress. The biological properties of IL-37 (IL-1F7) are mainly those of down-regulating inflammation.