Recombinant Saccharomyces cerevisiae Chitin deacetylase 1 (CDA1)

Product Specifications

Product Name Alternative

CDA1 ; L2142.2

Abbreviation

Recombinant Saccharomyces cerevisiae CDA1 protein

Gene Name

CDA1

UniProt

Q06702

Expression Region

25-301aa

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Target Sequence

SNGSTALMGEVDMQTPFPEWLTEFTNLTQWPGIDPPYIPLDYINLTEVPELDRYYPGQCPKISREQCSFDCYNCIDVDDVTSCFKLSQTFDDGPAPATEALLKKLRQRTTFFVLGINTVNYPDIYEHILERGHLIGTHTWSHEFLPSLSNEEIVAQIEWSIWAMNATGKHFPKYFRPPYGAIDNRVRAIVKQFGLTVVLWDLDTFDWKLITNDDFRTEEEILMDINTWKGKRKGLILEHDGARRTVEVAIKINELIGSDQLTIAECIGDTDYIERYD

Tag

C-terminal 6xHis-tagged

Type

Developed Protein

Source

Yeast

Field of Research

Signal transduction

Relevance

Hydrolyzes the N-acetamido groups of N-acetyl-D-glucosamine residues in chitin to form chitosan and acetate. Chitosan is a component of the spore wall

Purity

Greater than 95% as determined by SDS-PAGE.

Activity

Not test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

33.4 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.