Recombinant Human immunodeficiency virus 1 Protein Rev (rev) (P93S, X94V)

Product Specifications

Abbreviation

Recombinant Human immunodeficiency virus 1 rev protein (P93S, X94V)

Gene Name

Rev

UniProt

A0A0H4LST7

Expression Region

1-117aa (P93S, X94V)

Organism

Human immunodeficiency virus 1

Target Sequence

MAGRSGSTDEELLRAVRIINILYQSNPYPSTEGGTRQTRKNRRRRWRARQRQIRAICERILSSCVGRSTEPVPLQLPPLERLKLDCSEDCGTSVETGVGRPQISGESSVVLGSGTKN

Tag

N-terminal 6xHis-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Others

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

17.1 kDa

References & Citations

"Development of an HIV-1 Subtype Panel in China: Isolation and Characterization of 30 HIV-1 Primary Strains Circulating in China." Han J., Liu S., Guo W., Bao Z., Wang X., Li L., Liu Y., Zhuang D., Li H., Jia L., Gui T., Sui H., Li T., Li J. PLoS ONE 10:E0127696-E0127696 (2015)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.