Recombinant Severe acute respiratory syndrome coronavirus 2 Nucleoprotein (N) (L230F) (Active)
Product Specifications
Product Name Alternative
/
Abbreviation
Recombinant SARS-CoV-2 N protein (L230F) (Active)
Gene Name
N
UniProt
P0DTC9
Expression Region
1-419aa (L230F)
Organism
Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Target Sequence
MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQFESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
Tag
N-terminal 6xHis-tagged
Type
Active Protein & In Stock Protein & Coronavirus Protein
Source
E.coli
Field of Research
Microbiology
Endotoxin
Not test
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Yes
Bioactivity
①Measured by its binding ability in a functional ELISA. Immobilized N (CSB-EP3325GMY (M9) ) at 2 μg/ml can bind N Mouse Monoclonal Antibody (CSB-MA33255A2m), the EC50 of N Mouse Monoclonal Antibody is 7.401-12.67 ng/ml. ②Measured by its binding ability in a functional ELISA. Immobilized N (CSB-EP3325GMY (M9) ) at 2 μg/ml can bind N Recombinant Antibody (CSB-RA33255A1GMY), the EC50 of N Recombinant Antibody is 4.854-7.721 ng/ml.
Form
Lyophilized powder
Buffer
Lyophilized from 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
49.7 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length
Available Sizes
Curated Selection
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