Recombinant Lampetra fluviatilis NADH-ubiquinone oxidoreductase chain 1 (MT-ND1)
Selected Variant
CSB-CF015076LNM (A4)-01
Size: 20 µg
Product Specifications
Product Name Alternative
NADH dehydrogenase subunit 1 MTND1, NADH1, ND1
Abbreviation
Recombinant Lampetra fluviatilis MT-ND1 protein
Gene Name
MT-ND1
UniProt
O21069
Expression Region
1-321aa
Organism
Lampetra fluviatilis (European river lamprey) (Petromyzon fluviatilis)
Target Sequence
MLVMLTSTLILVLMVLLAVAFLTMVERKTLGYMQLRKGPNVVGFMGLLQPIADGVKLFLKEPVWPTAASPALFIAAPIMALTLALSLWMFIPMPQSISTINLTLLVILAISSLSVYASLGSGWASNSKYALIGALRAVAQTISYEVSLGLILLCLIILTGGFSLQAFIYTQEHTWFLLSSWPLAAMWFVSTLAETNRTPFDLTEGESELVSGFNVEYAGGPFALFFLAEYSNILFMNTLTAIMFLGPLGPNNLNILPIINVMMKATPLIILFLWIRASYPRFRYDQLMHLMWKNFLPLNLALFTLQLSLAVSLGGAGVPQM
Tag
N-terminal 10xHis-SUMO-tagged
Type
CF Transmembrane Protein & Developed Protein
Source
In vitro E.coli expression system
Field of Research
Others
Relevance
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone
Endotoxin
Not test
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Function
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity) .
Molecular Weight
53.8 kDa
References & Citations
"The main features of the craniate mitochondrial DNA between the ND1 and the COI genes were established in the common ancestor with the lancelet." Delarbre C., Barriel V., Tillier S., Janvier P., Gachelin G. Mol. Biol. Evol. 14:807-813 (1997)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length
Available Sizes
Price
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