ProBlock™ Gold Mammalian Protease Inhibitor Cocktail [100X]

ProBlock™ Gold mammalian protease inhibitor cocktail contains optimized concentrations of various mammalian protease inhibitors, which provide excellent inhibition of protease activities during protein purification from mammalian cells and tissues. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine, cysteine and specific inhibitors for mammalian proteases, such as aminopeptidases. A 1X concentration of ProBlock™ Gold Mammalian inhibits over 90% of protease activities.Some proteins require divalent cations (Ca2+, Mg2+or Mn2+) for their biological activity and the presence of chelating agents may be detrimental to the protein’s activity. Chelators, such as EDTA, inhibit the purification of proteins by immobilized metal affinity chromatography.ProBlock™ Gold Mammalian is supplied with additional, optional EDTA and 1,10-Phenanthroline monohydrate which are provided for optimal inhibition of metalloproteases and may be added in the extraction buffer or lysate as needed.Benefits:Specifically optimized for mammalian proteasesGreater Range of Inhibition – 95%+Better than tablets – the ready-to-use, refrigerator-stable, 100X liquid format is more effective and easier to use than traditional tablet products; just pipette exactly the amount you need!No proprietary ingredients – formulation contains AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A, and PMSFMultiple package sizesTwo popular formulations – regular formulation includes separate vials of EDTA and 1,10-Phenanthroline monohydrate solutions for optional metalloprotease inhibition; EDTA-free and 1,10-Phenanthroline-free formulations ensures compatibility with isoelectric focusing