Mix-n-Stain™ CF®750 Antibody Labeling Kit

Mix-n-Stain™ CF®750 Antibody Labeling Kit is a near-infrared fluorescent dye that is the brightest fluorescent dye available in its spectrum. Antibody conjugates prepared from CF®750 have by far the best signal-to-noise ratio than antibody conjugates prepared from other spectrally similar dyes.Mix-n-Stain™ antibody labeling kitsare a revolutionary antibody labeling technology that allows you to label your primary antibody with biotin or one of our superior CF®dyes in 30 minutes without the need for a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. CF®dyes are highly water soluble, small organic dyes designed for labeling proteins and nucleic acids. Many of our CF®dyes are brighter and more photostable than competing dyes. Click the link for a complete list of more than 20 GoldBioCF®antibody labeling kits.Note: We do not recommend using CF®750 labeled primary antibodies for use with the APC-Cy7 channel. The excitation wavelength for the APC-Cy7 channel is suboptimal for CF®750 and direct immunofluorescence staining using primary antibodies labeled with CF®750 generally results in very low signal.Technical SummaryAbs/Em Maxima: 755/777 nmExcitation laser line: 633 nm, 635 nm, 680 nm or 685 nmExtinction Coefficient: 250,000MW: ~3009 g/molKit ComponentsDyeMix-n-Stain™ 10X reaction bufferMix-n-Stain™ antibody storage bufferUltrafiltration vial