TNF-α, Murine

GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!Recombinant Murine Tumor Necrosis FactorTNF-α, TNF-alphaCachectin, CachexinTumor necrosis factor (or TNF-α), also called cachectin, is produced by neutrophils, activated lymphocytes, macrophages, NK-cells, LAK-cells, astrocyte endothelial cells, smooth muscle cells and some transformed cells. TNF can occur as a secreted, soluble form or as a membrane-anchored form, both of which are biologically active. Mouse TNF occurs as a membrane-anchored form. The naturally occurring form of TNF is glycosylated, but nonglycosylated recombinant TNF has comparable biological activity. The biologically active native form of TNF is reportedly a trimer. Human and murine TNF has approximately 79% homology at the amino acid level and there is cross-reactivity between the two species. Two types of receptors for TNF, p55 and p75, have been described and virtually all cell types studied show the presence of one or both of these receptor types.Formulation: Sterile filtered white lyophilized powder. Purified and tested for use in cell culture.Sequence: MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIALMW: ~17.3 kDa (157 amino acid) Purity: > 98 % by SDS-PAGE and HPLC analyses.Biological Activity: Fully biologically active when compared to standard. The ED50as determined by a cytotoxicity assay using murine L929 cells is less than 0.1 ng/ml, corresponding to a specific activity of >1.0 x 107IU/mg in the presence of actinomycin D.Accession Number:P06804MGI ID:104798Source:Escherichia coli