TNF-α (His), Human

GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!Recombinant Human Tumor Necrosis Factor, HisTNF-α, TNF-alphaCachectin, CachexinTumor necrosis factor (or TNF-α), also called cachectin, is produced by neutrophils, activated lymphocytes, macrophages, NK-cells, LAK-cells, astrocyte endothelial cells, smooth muscle cells and some transformed cells. TNF occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally occurring form of TNF is glycosylated, but nonglycosylated recombinant TNF has comparable biological activity. The biologically active native form of TNF is reportedly a trimer. Human and murine TNF has approximately 79% homology at the amino acid level and there is cross-reactivity between the two species. Two types of receptors for TNF, p55 and p75, have been described and virtually all cell types studied show the presence of one or both of these receptor types.TNF-α, His has an identical amino acid sequence to Human TNF-α, but with 6x-His at the N-terminus for protein purification (see Additional Information for sequence) .Sterile Filtered White lyophilized powder. Purified and tested for use in cell culture.Source:Escherichia coliMW: ~18.3 kDa (157 amino acid plus 6x His at N-terminus) Purity: >97% by SDS-PAGE and HPLC analyses