DEFB3, Murine

GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!Recombinant MurineDefensin, Beta 3BD3, DEFB-3, MBD-3, DEFB103AMurine beta-defensin 3 (DEFB3) is an antimicrobial peptide that contributes to the innate and adaptive immune systems and is active against gram-negative and gram-positive bacteria, fungi, and viruses. Like the other β-defensins, DEFB3 is a small protein that contains a motif consisting of six cysteine residues which form three intramolecular disulfide bridges. It is expressed in epithelial tissue of many organs, including the skin and lungs, and in leukocytes. Expression of DEFB3 is induced by proinflammatory cytokines such as IL1B and IFNG. Expression of murine DEFB3 was found to be downregulated in the epidermis by psychological stress due to decreased glucocorticoid production and subsequent decreased defensin-encapsulating lamellar body secretion. DEFB3 is a cationic peptide and interacts with the membranes of invading microbes, which are negatively charged due to the presence of lipopolysaccharides (LPS) or lipoteichoic acid (LTA) . LTA and LPS have higher affinity for DEFB3 than for Ca+2and Mg+2ions. The larger defensin molecule displaces the smaller ion, changing the membrane structure and affecting the stability of the membrane; this can lead to the formation of pores and subsequent depolarization or lysis. Inhibition of DEFB3 by high salt concentration may play a role in the pathogenesis of cystic fibrosis.Sterile Filtered White lyophilized powder. Purified and tested for use in cell culture.Source:Escherichia coliMW: ~4.6 kDa (41 amino acids) Purity: >95% by SDS-PAGE and HPLC analyses.Biological Activity: Fully biologically active when compared to standard. The ED50as determined by anti-microbial activity againstE. coliis less than 20 μg/ml, corresponding to a specific activity of >50 IU/mg.