DEFB2, Murine

GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!Recombinant Murine Defensin, Beta 2BD2, DEFB-2, MBD-2Murine beta-defensin 2 (DEFB2) is an antimicrobial peptide that contributes to the innate immune system and is active against gram-negative bacteria, fungi, and viruses. It also contributes to the adaptive immune system through recruitment of leukocytes to sites of infection through chemotaxis. Like the other β-defensins, DEFB2 is a small protein that contains a motif consisting of six cysteine residues which form three intramolecular disulfide bridges. It is expressed in the epithelia of skin and organs in the genitourinary tract, as well as in monocytes and macrophages. Expression is induced by 1,25-dihydroxyvitamin D3, LPS, and proinflammatory cytokines, such as IL1B and IFNG, and downregulated by the anti-inflammatory steroid dexamethasone. DEFB2 expression is also induced by exposure to low M.W. hyaluronic acid fragments, which is common following inflammation or tissue injury. DEFB2 is a cationic peptide that disrupts the membranes of invading microbes, which are negatively charged due to the presence of lipopolysaccharides (LPS) or lipoteichoic acid (LTA) . In mice that have had theDefb2gene silenced, inducible NO synthase expression was elevated. DEFB2 works in combination with DEFB3 to contribute to resistance against corneal infection. Inhibition of DEFB2 by high salt concentration may play a role in the pathogenesis of cystic fibrosis.Sterile Filtered White lyophilized powder. Purified and tested for use in cell culture.Source:Escherichia coliMW: ~5.5 kDa (51 amino acids) Purity: >98% by SDS-PAGE and HPLC analyses.Biological Activity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using immature human dendritic cells is in a concentration range of 10-100 ng/ml.