DEFB1, Murine

GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!Recombinant MurineDefensin, Beta 1BD1, DEFB-1, MBD-1Murine beta-defensin 1 (DEFB1) is an antimicrobial peptide that contributes to the innate and adaptive immune systems and is active against bacteria, fungi, and viruses. Like the other β-defensins, DEFB1 is a small protein that contains a motif consisting of six cysteine residues which form three intramolecular disulfide bridges. It is constitutively expressed in epithelial tissue, mainly of the respiratory and genitourinary tract, as well as in monocytes, macrophages, and dendritic cells. DEFB1 is a cationic peptide and interacts with the membranes of invading microbes, which are negatively charged due to the presence of lipopolysaccharides (LPS) or lipoteichoic acid (LTA) . LTA and LPS have higher affinity for DEFB1 than for Ca+2and Mg+2ions. The larger defensin molecule displaces the smaller ion, changing the membrane structure and affecting the stability of the membrane; this can lead to the formation of pores and subsequent depolarization or lysis. The primary role of DEFB1 is believed to be prevention of microbial colonization on epithelia. Transgenic mice lacking theDefb1gene showed an increased load ofStaphylococcusspecies in the bladder, suggesting a role for DEFB1 in resistance to urinary tract infection. Inhibition of DEFB1 by high salt concentration may play a role in the pathogenesis of cystic fibrosis.Sterile Filtered White lyophilized powder. Purified and tested for use in cell culture.Source:Escherichia coliMW: ~5.2 kDa (48 amino acids) Purity: >97% by SDS-PAGE and HPLC analyses.Biological Activity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using CD34+ dendritic cells is in a concentration range of 100-1000 ng/ml.