SERPINF1, Human

GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!Recombinant Human Serpin Peptidase Inhibitor, clade F, member 1Pigment Epithelium Derived Factor; PEDFSERPINF1alpha-2 antiplasminSerpin Peptidase Inhibitory, clade F (SERPINF1), previously called Pigment epithelium-derived factor (PEDF), is encoded by the SERPINF1 gene in humans and found in vertebrates. It is a secreted phosphoglycoprotein that belongs to the clade F subfamily, serpin superfamily of proteinase inhibitors. SERPINF1 is a noninhibitory serpin with neurotrophic, anti-angiogenic, and anti-tumorigenic properties. It is synthesized as a 418 amino acid, ~50 kDa precursor that contains a 19 amino acid signal sequence and a 399 amino acid mature region that shows a pyroglutamate at Gln20. Like other serpins, it contains three β-sheets, 810 α-helices, and a C-terminal RCL (reactive center loop) . Unlike other serpins with Ser protease inhibiting activity, SERPINF1 has induces extensive neuronal differentiation in retinoblastoma cells, inhibiting of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. SERPINF1 is researched as a therapeutic candidate for treatment of such conditions as choroidal neovascularization, heart disease, and cancer.Sterile Filtered White lyophilized powder. Purified and tested for use in cell culture.Source:Escherichia coliMW: ~44.4 kDa (399 amino acids) Purity: >97% by SDS-PAGE and HPLC analyses.Biological Activity: Fully biologically active when compared to standard. The ED50as determined by its ability to enhance the adhesion of human Saos2 cells to bovine Collagen I coated plate is less than 2 ng/ml, corresponding to a specific activity of >5.0x105IU/mg.