CSF1 (M-CSF), Murine

GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!Recombinant Murine Colony Stimulating Factor 1 (Macrophage) Macrophage Colony Stimulating Factor, M-CSFRecombinant Murine Colony Stimulating Factor 1 (Macrophage) (CSF1), formerly called Macrophage Colony Stimulating Factor (M-CSF), is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It is produced by osteoblasts (as a result of endocrine stimulation by parathyroid hormone) exerts paracrine effects on osteoclasts and can interact with CSF1R. CSF1 is a four α-helical bundle cytokine and its active form is found extracellularly as a disulfide-linked homodimer. Four transcript variants encoding three different isoforms have been reported for CSF1 gene. Although forms may vary, all of them contain the N-terminal 150 amino acid portion that is necessary and sufficient for interaction with the receptor. The first 229 amino acids of mature mouse CSF1 shares 87%, 83%, 82% and 81% sequence identity with corresponding regions of rat, dog, cow and human CSF1, respectively. Human CSF1 is active in the mouse, but mouse CSF1 is reported to be species-specific.Formulation: Sterile filtered white lyophilized powder. Purified and tested for use in cell culture.Sequence: KEVSEHCSHM IGNGHLKVLQ QLIDSQMETS CQIAFEFVDQ EQLDDPVCYL KKAFFLVQDI IDETMRFKDN TPNANATERL QELSNNLNSC FTKDYEEQNK ACVRTFHETP LQLLEKIKNF FNETKNLLEK DWNIFTKNCN NSFAKCSSRD VVTKPDCNCL YPKATPSSDP ASASPHQPPA PSMAPLAGLA WDDSQRTEGS SLLPSELPLR IEDPGSAKQR PPRSTCQTLEMW: ~52.0 kDa (a disulfide-linked homodimer consisting of two 230 amino acid polypeptide chains) .Purity: >95% by SDS-PAGE and HPLC analyses.Biological Activity: Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using murine M-NFS-60 cells is less than2.0 ng/ml, corresponding to a specific activity of >5.0 x 109IU/mg.Accession Number:Q3U4F9MGI ID:1339753Source:Escherichia coli