CSF1 (M-CSF), Human

GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!Recombinant Human Colony Stimulating Factor 1 (macrophage) Macrophage Colony Stimulating Factor; M-CSFCSF1Colony Stimulating Factor 1 (CSF1), previously called Macrophage Colony Stimulating Factor (M-CSF), is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It is produced by osteoblasts (as a result of endocrine stimulation by parathyroid hormone), exerts paracrine effects on osteoclasts and can interact with CSF1R. CSF1 is a four α-helical bundle cytokine and its active form is found extracellularly as a disulfide-linked homodimer. Four transcript variants encoding three different isoforms have been reported for CSF1 gene. Although forms may vary, all of them contain the N-terminal 150 amino acid portion that is necessary and sufficient for interaction with the receptor. The first 223 amino acid of mature human CSF1 shares 88%, 86%, 81% and 74% sequence identity with corresponding regions of dog, cow, mouse and rat CSF1, respectively. Human CSF1 is active in the mouse, but mouse CSF1 is reported to be species-specific.Formulation: Sterile filtered white lyophilized powder. Purified and tested for use in cell culture.Sequence: EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQG HERQSEGSMW: ~36.8 kDa (2x 158 amino acids) Purity: >95% by SDS-PAGE and HPLC analyses.Biological Activity: Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 1 ng/ml, corresponding to a specific activity of >1.0x106IU/mg.Accession Number:P09603HGNC ID:2432Source:Escherichia coli