Proteinase K, RNase/DNase free

Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins. It cleaves at the carboxylic acid side of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. This enzyme was designated proteinase K because of its ability to hydrolyze keratin. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9.Proteinase K is typically used at a concentration of 50-100 μg/ml. It is active with or without the presence of SDS, urea, EDTA or various metal ions, but the activity of proteinase K can be increased by adding the denaturing agents and the structure of proteinase K can be stabilized by addition of Ca2+. Proteinase K is inactivated by heating to 95°C for 10 minutes or using an inhibitor such as PMSF, AEBSF or DFP.GoldBio's Proteinase K undergoes two sterile filtration steps during the separation and purification processes. The final product is further analyzed by plate culture to ensure the removal of all living organisms was 100% effective.Unit Definition: One unit is defined as the amount of enzyme that will liberate 1.0 μmol tyrosine (Folin-positive amino acid) from casein per minute at 37°C, pH 7.5.Request a Bulk QuoteNeed larger quantities for your research or production runs? Usethis form to request a custom bulk quoteand secure the best value for your lab. Your account manager will follow up with you promptly to provide tailored pricing and support for your order.ApplicationsDigestion of unwanted proteinsRemoval of endotoxins bound to cationic proteins such as lysozyme and RNaseARemoval of nucleases for in situ hybridizationPrion research with respect to TSE (transmissible spongiform encephalopathies) Protease footprinting Mitochontrial isolationIsolation of genomic DNAIsolation of cytoplasmic RNAIsolation of highly native DNA or RNA