Lysozyme, Egg White

GoldBio’s Lysozyme (Egg White) is a high-activity, purified enzyme widely used in molecular biology and enzymology research. This lyophilized powder is highly effective at disrupting the cell walls of Gram-positive bacteria and can also aid in Gram-negative lysis when combined with membrane-permeabilizing treatments.It is a classic model enzyme for kinetics, catalytic mechanism, and structural biology studies, including protein crystallography, making it a cornerstone tool in both teaching and advanced research laboratories.Lysozyme is soluble in aqueous buffers and is rigorously quality-controlled, providing reproducible performance across DNA, RNA, and protein extraction workflows, enzymatic activity assays, and structural-function experiments.Gram-positive cells have an increased susceptibility to the actions of lysozyme since gram-positive cells have a higher quantity of peptidoglycan in the cell wall. Gram-negative cells are less vulnerable, but can still be hydrolyzed in the presence ofEDTA.Unit Definition: 1 FIP unit of lysozyme is the amount of Enzyme that hydrolyzes under the standard conditions 1 mmole of aMicrococcus lysodeikticusper minute.Common Applications (Click each for more information) Lysis of Gram-Positive and Gram-Negative Bacterial Cell WallsPurpose: To disrupt bacterial cell walls for DNA, RNA, or protein extraction.How It Works: Lysozyme hydrolyzes the β (1→4) glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in peptidoglycan, weakening the wall and causing osmotic lysis. Gram-positive bacteria are especially susceptible; Gram-negative bacteria typically require additional agents (e.g., EDTA) to permeabilize the outer membrane.Applications: Used in genomic DNA extraction, plasmid minipreps, and bacterial protein extraction.Sambrook, J., & Russell, D. W. (2001) .Molecular Cloning: A Laboratory Manual (3rd ed.) . Cold Spring Harbor Laboratory Press.Enzymatic Lysis in Protein Purification ProtocolsPurpose: To prepare cell lysates for recombinant protein purification.How It Works: Used together with detergents and nucleases to ensure efficient breakdown of bacterial cell walls while preserving protein integrity.Applications: Widely used in His-tag protein purification, enzyme studies, and proteomics workflows.Walls, D., & Loughran, S. T. (2007) . Protein Chromatography: Methods and Protocols, 681, 15–22.Model Enzyme in Enzyme Kinetics and Structure-Function StudiesPurpose: To explore enzyme-substrate interactions, catalytic rates, and structure-function relationships.How It Works: Lysozyme’s extensively studied structure makes it a model system for enzymology education and research, often serving as the "textbook enzyme."Applications: Kinetics labs, inhibitor screening, and catalytic mechanism studies.Vocadlo, D. J., Davies, G. J., Laine, R., & Withers, S. G. (2001) .Nature, 412 (6849), 835–838.Standardization of Muramidase Activity AssaysPurpose: To measure lysozyme activity for enzyme QC, inhibitor testing, or standardization.How It Works: Lysozyme activity is measured via the lysis ofMicrococcus lysodeikticuscells, monitoring turbidity reduction at 450 nm.Applications: Used in enzyme validation, commercial product QC, and inhibitor screening.Shugar, D. (1952) .Biochimica et Biophysica Acta, 8, 302–309.Structural Biology and Crystallography StudiesPurpose: To investigate protein folding, crystallization methods, and structure-based drug discovery.How It Works: Hen egg-white lysozyme is one of the most widely crystallized proteins, making it a model for developing crystallographic techniques and testing protein-ligand interactions.Applications: X-ray crystallography, protein folding studies, and drug binding assays.McPherson, A. (1982) .Preparation and Analysis of Protein Crystals. Wiley.Key Benefits:Effective lysis of Gram-positive bacteria:Facilitates DNA, RNA, and protein extraction workflows. Gram-negative bacteria typically require lysozyme in combination with membrane-permeabilizing agents.High enzymatic activity:Validated potency ensures reliable performance in activity assays and bacterial lysis under appropriate conditions.Well-characterized structure:Ideal for enzymology education, mechanistic studies, and structural biology.Purified natural source:Derived from hen egg white and standardized for reproducible laboratory performance.Versatile applications:Widely used in lysis protocols, enzymatic activity assays, protein crystallography, and structure-function studies.Working ConcentrationMake stock solutions at 10 mg/ml in 10mM Tris-HCl, pH 8.0Working concentration should be between0.1-1.0 mg/ml.