DNase I, Bovine Pancreas (Lysate Tested), >500 Kunitz U/mg

DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and phosphate group at the 5' position. The optimum pH is 7.8.DNase I is activated by bivalent metals, and inhibited by chelating agents such as EDTA and SDS. DNase I can be stabilized against proteolytic digestion by 5mM Ca2+.Product SpecificationsDNase IDeoxyribonuclease IDNase I, Bovine Pancreas, >500 Kunitz U/mgMOLECULAR BIOLOGY GRADEUnit Definition:One Kunitz Unit will produce an A260of 0.001 per minute at pH 5.0 at 25°C using DNA, type I or III as the substrate.