Goof-Proof™ Universal Probe Master Mix with ROX

Goof-Proof™ Universal Probe Master Mix with ROX is a high-performance 2X master mix for fluorescent probe-based PCR applications such as SNP genotyping and quantitation. Goof-Proof™ has broad instrument compatibility, can be used in both standard and fast protocols and shows a high degree of consistency in both singleplex and multiplex reactions. The Goof-Proof™ Universal Probe Master Mix is perfectly designed for all fluorescent probe technologies, including displacement probes such as Molecular Beacon and hydrolysis probes like TaqMan®.Figure 1: Reaction afteradding template with1X Goof-Proof™ TrackingBufferKit componentsGoldBio HotStart Taq DNA Polymeraseand dNTPs in an optimized buffer suitable for high qPCR sensitivity and multiplex reactions. Primers, probe and template not included.Goof-Proof™ Tracking Buffer -The 40X Tracking Buffer contains an inert blue dye. Adding the tracking buffer to the DNA template makes it “Goof-Proof” to track which reactions have had template added to the PCR reactions. Alternatively, Goof-Proof™ Tracking Buffer can be added to the master mix to distinguish wells with reaction buffer from empty wells. Tracking Buffer is not required for the reaction to function, but is designed to help alleviate mistakes.ROX Reference Dye -ROX is necessary for accurate Ct determination from well to well with certain instruments (such as some ABI and Stratagene PCR instruments, refer to your instrument instructions to see if ROX is required) . ROX may add noise to melt curve analysis, which could be mistaken for real peaks. In the case of unexpected peaks, un-check “ROX” in the “Passive Reference Dye” box in the system software to prevent data collection from the ROX fluorescence channel and re-analyze the data.Note: We also offerGoof-Proof™ Universal Probe Master Mix (Catalog # G-710) without ROX for instruments that do not require the ROX reference dye.