PSoup Plasmid

Introducing a new family ofAgrobacteriumhelper plasmids, pSuperAgro™! These plasmids can greatly enhance gene transfer into various species of plants duringAgrobacterium-mediated transformation. This increased efficiency provides a significant time and labor savings.Sold as "Transformation Ready" DNA and offered at a low price to encourage rapid adoption of this exciting new technology. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research.pSuperAgro™ v4 was designed to include AcdS and GabT activity, driven by a singlelacpromoter, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.AcdS activity is beneficial for higher callus induction frequency and also suppresses callus browning phenomena. GabT activity decreases GABA content locally in plant cells and induces higher shoot regeneration, decreasing the number of cotyledons used for transformation and reducing the time and labor required for transformation. This plasmid has been shown to be efficacious in both Hardy Pampas Grass (E. Ravennae) and Tomato plants (S. Lycopersicum) .pSuperAgro™ v4tet has been modified to provide tetracycline resistance instead of the original pSuperAgro™ V4’s gentamicin resistance. This allows the helper plasmid to be used in conjunction with our other GoldBioAgrobacteriumstrains, including the widely used GV3101 strain.These products are sold under license by GoldBio and require a signed agreement before fulfillment. See license details below in the technical documents.BenefitsEthylene evolution suppressionReduced GABA contentHigher callus induction frequencyReduced callus browningInduces higher shoot regenerationIncreased transient and stable transformation efficienciesProduct SpecificationsConcentration: 0.02 μg/μL in TEVolume: 50 μLBackbone size: 7480 bpVector type: Helper vectorPromoter: LacBacterial Resistance (s) : TetracyclineCopy number: Low CopyVector MapSequenceGoldBio Vector Map and Sequence for pSuperAgro™ v4tet