AGL-1 Agrobacterium Chemically Competent Cells

AGL-1 (pSoup-P19) electrocompetentAgrobacteriumcells are a highly efficient system for genetic transformation in various plant species. This strain is derived from the C58 RecA-typeAgrobacteriumstrain and carries the rifampicin, carbenicillin, and streptomycin resistance genes for selection purposes. The Ti plasmid pTiBo542DT-DNA used in this system has a deleted T-DNA region, and instead contains a binary vector that restores the T-DNA binary system function.The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and further improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign. These features help to enhance theAgrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.Kit ComponentsCompetent Cells1 x 12 mL Recovery Media1 x 10 µL Control Plasmid (pCAMBIA1391z Control, 500 pg/µL)