EHA105 Agrobacterium Chemically Competent Cells

GoldBio’s AGL-1Agrobacteriumchemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in itsrecA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted from the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant S genome. Therefore, our system is often used forAgrobacterium-mediated transformation in mono and dicotyledonous species such asArabidopsis thaliana, maize, and other plants.AGL-1 chemically competent cells arefree of animal-derived productsand grown with animal-free media.Kit ComponentsCompetent Cells1 x 12 mL Recovery Media1 x 25 µL Control Plasmid (pCAMBIA1391z Control, 10 ng/µL) Reagents Needed for One ReactionAGL-1 Chemically CompetentAgrobacterium: 50 µLDNA (pCAMBIA1391z Control, 10 ng/µL) : 5 µLRecovery medium: 1 mL