EHA105 (pSuperAgro™ v4tet) Agrobacterium Electrocompetent Cells

GoldBio’s GV3101Agrobacteriumchemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) harboring the gentamicin resistance. A functional T-DNA binary system can be built using our GV3101 strains as the T-DNA region has been deleted in the Ti plasmid and instead has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Our system is often used forAgrobacterium-mediated transformation in mono and dicotyledonous species such asArabidopsis thaliana, tobacco, potato, soybeans and corn.GV3101 chemically competent cells arefree of animal-derived productsand grown with animal-free media.Kit ComponentsCompetent Cells1 x 12 mL Recovery Media1 x 25 µL Control Plasmid (pCAMBIA1391z Control, 10 ng/µL) Reagents Needed for One ReactionGV3101 chemically competentAgrobacterium: 50 µLDNA (pCAMBIA1391z Control, 10 ng/µL) : 5 µLRecovery medium: 1 mL