C58C1 (pSuperAgro™ v4) Agrobacterium Chemically Competent Cells

GoldBio’s LBA4404 ElectroCompetentAgrobacteriumcells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbor a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octoprine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes. Our LBA4404 strain can be used in genetic transformation of tomato, tobacco and other plants.pSuperAgro™v4 was designed to include AcdS and GabT activity, driven by a singlelacpromotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.These products are sold under license by GoldBioand require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.Kit ComponentsCompetent Cells1 x 12 mL Recovery Media1 x 10 µL Control Plasmid (pCAMBIA1391z Control, 500 pg/µL) Reagents Needed for One ReactionLBA4404 (pSuperAgro™ v4) ElectroCompetentAgrobacterium: 25 µLDNA (pCAMBIA1391z Control, 500 pg/µL) : 1 µLRecovery medium: 1 mL