C58C1 (pSuperAgro™ v4) Agrobacterium Electrocompetent Cells

GoldBio’s C58C1 Chemically CompetentAgrobacteriumcells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. C58C1 has the chromosomal backbone of C58 but it has been cured of the Ti plasmid pTiC58. Our C58C1 strain harbors streptomycin and rifampicin resistance genes. C58C1 Competent cells are optimized for genetic transformation of plants such as Arabidopsis but can be used in many other plants.pSuperAgro™v4 was designed to include AcdS and GabT activity, driven by a singlelacpromotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.These products are sold under license by GoldBioand require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.Kit ComponentsCompetent Cells1 x 12 mL Recovery Media1 x 25 µL Control Plasmid (pCAMBIA1391z Control, 10 ng/µL) Reagents Needed for One ReactionC58C1 (pSuperAgro™ v4) Chemical CompetentAgrobacterium: 50 µLDNA (pCAMBIA1391z Control, 10 ng/µL) : 5 µLRecovery medium: 1 mL