EHA105 (pSuperAgro™ v4tet) Agrobacterium Chemically Competent Cells

GoldBio’s AGL-1Agrobacteriumchemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in itsrecA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted from the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant S genome. Therefore, our system is often used forAgrobacterium-mediated transformation in mono and dicotyledonous species such asArabidopsis thaliana, maize, and other plants.pSuperAgro™v4 was designed to include AcdS and GabT activity, driven by a singlelacpromotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.These products are sold under license by GoldBioand require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.Kit ComponentsCompetent Cells1 x 12 mL Recovery Media1 x 25 µL Control Plasmid (pCAMBIA1391z Control, 10 ng/µL) Reagents Needed for One ReactionAGL-1 (pSuperAgro™ v4) Chemically CompetentAgrobacterium: 50 µLDNA (pCAMBIA1391z Control, 10 ng/µL) : 5 µLRecovery medium: 1 mL