EHA105 (pSuperAgro™ v4tet) Agrobacterium Chemically Competent Cells

GoldBio’s GV3101Agrobacteriumchemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) harboring the gentamicin resistance. A functional T-DNA binary system can be built using our GV3101 strains as the T-DNA region has been deleted in the Ti plasmid and instead has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Our system is often used forAgrobacterium-mediated transformation in mono and dicotyledonous species such asArabidopsis thaliana, tobacco, potato, soybeans and corn.pSuperAgro™v4 was designed to include AcdS and GabT activity, driven by a singlelacpromotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants. pSuperAgro™v4tet has been modified to provide tetracycline resistance instead of the original pSuperAgro™ V4’s gentamicin resistanceThese products are sold under license by GoldBioand require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.Kit ComponentsCompetent Cells1 x 12 mL Recovery Media1 x 25 µL Control Plasmid (pCAMBIA1391z Control, 10 ng/µL) Reagents Needed for One ReactionGV3101 (pSuperAgro™ v4tet) chemically competentAgrobacterium: 50 µLDNA (pCAMBIA1391z Control, 10 ng/µL) : 5 µLRecovery medium: 1 mL