C58C1 Agrobacterium Chemically Competent Cells

GoldBio’s EHA105 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene. EHA105 is ideal for genetic transformation of rice, tobacco and several other plants.pSuperAgro™v4 was designed to include AcdS and GabT activity, driven by a singlelacpromotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants. pSuperAgro™v4tet has been modified to provide tetracycline resistance instead of the original pSuperAgro™ V4’s gentamicin resistanceThese products are sold under license by GoldBioand require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.GoldBio’s EHA105Agrobacteriumstrain was generated, and primary clone supplied by Dr. Elizabeth Hood.Kit ComponentsCompetent Cells1 x 12 mL Recovery Media1 x 25 µL Control Plasmid (pCAMBIA1391z Control, 10 ng/µL) Reagents Needed for One ReactionEHA105 (pSuperAgro™ v4tet) Chemical CompetentAgrobacterium: 50 µLDNA (pCAMBIA1391z Control, 10 ng/µL) : 5 µLRecovery medium: 1 mL