EHA105 (pSoup-P19) Agrobacterium Chemically Competent Cells

AGL-1 (pSoup) electrocompetentAgrobacteriumcells are a highly efficient system for genetic transformation in various plant species. This strain is derived from the C58 RecA-typeAgrobacteriumstrain and carries the rifampicin, carbenicillin, and streptomycin resistance genes for selection purposes. The Ti plasmid pTiBo542DT-DNA used in this system has a deleted T-DNA region, and instead contains a binary vector that restores the T-DNA binary system function.The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids, helping to enhance theAgrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.Kit ComponentsCompetent Cells1 x 12 mL Recovery Media1 x 10 µL Control Plasmid (pCAMBIA1391z Control, 500 pg/µL) Reagents Needed for One ReactionAGL-1 (pSoup) ElectroCompetentAgrobacterium: 25 µLDNA (pCAMBIA1391z Control, 500 pg/µL) : 1 µLRecovery medium: 1 mL