AGL-1 (pSoup-P19) Agrobacterium Chemically Competent Cells

GoldBio’s EHA105 (pSoup-P19) chemically competentAgrobacteriumcells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, chloramphenicol, and streptomycin, providing versatility in selection and antibiotic resistance EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene.The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids, increasing the range of applications for the EHA105 (pSoup) system. The p19 protein is derived from tomato bush dwarf virus and further improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign. These competent cells are suitable forAgrobacterium-mediated transformation of dicots such asArabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.GoldBio’s EHA105Agrobacteriumstrain was generated, and primary clone supplied by Dr. Elizabeth Hood.Kit ComponentsCompetent Cells1 x 12 mL Recovery Media1 x 25 µL Control Plasmid (pCAMBIA1391z Control, 10 ng/µL)