EHA105 (pSoup) Agrobacterium Chemically Competent Cells

GoldBio’s AGL-1 (pSoup-P19) chemically competentAgrobacteriumcells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, carbenicillin, and streptomycin, providing versatility in selection and antibiotic resistance. The Ti plasmid pTiBo542DT-DNA has been engineered to have the T-DNA region deleted, which can be complemented with a binary vector containing the missing T-region to restore the T-DNA binary system function. This feature enables efficient and precise transformation of plant genomes.The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign genes and increasing the range of applications for the AGL-1 (pSoup-P19) system. These competent cells are suitable forAgrobacterium-mediated transformation of dicots such asArabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.Kit ComponentsCompetent Cells1 x 12 mL Recovery Media1 x 25 µL Control Plasmid (pCAMBIA1391z Control, 10 ng/µL) Reagents Needed for One ReactionAGL-1 (pSoup-P19) chemically competentAgrobacterium: 50 µLDNA (pCAMBIA1391z Control, 10 ng/µL) : 5 µLRecovery medium: 1 mL