JM109 Chemically Competent E. coli Cells

GoldBio’s BL21 Chemically CompetentE. colicells are a good, universal strain of cell that is gives great transformation for protein expression and induction, and is especially useful for non-T7 vectorsprotein expression systems. BL21 chemically competent cells feature a widely used host background, and are deficient in both lon (1) and ompT proteases. In addition, BL21 Chemically CompetentE. colicells are resistant to phage T1 (fhuA2) .BL21 chemically chemically competent cells arefree of animal-derived productsand grown with animal-free media.BL21 HighlightsFree of all Animal productsHigh plasmid stability and decreased plasmid transferNo DNA cytosine methylation means better restriction enzyme digestionNo restriction/methylation systemGalactose deficiency and ideal for selective systemsPerfect for maltose-based T7-based systemsSensitive to phage λ (No T7 polymerase present) Ideal for stable protein expression with low protease activity.Kit ComponentsCompetent Cells1 x 12 mL Recovery Media1 x 10 µL Control Plasmid (pUC19 Control, 500 pg/µL) Reagents Needed for One ReactionBL21 chemically competent cells: 50 µLDNA (orpUC19 Control, 500 pg/µL) : 2 µLRecovery medium: 1 mL