GB5-alpha™ Chemically Competent E. coli Cells

GoldBio’s GB5-alpha™ Chemically CompetentE. colicells are equivalent to DH5-alpha cells and are high efficiency cells suitable for transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning.GB5-alpha™ chemically competent cells arefree of animal-derived productsand grown with animal-free media.GB5-alpha™ Chemically competentE. colicells are equivalent to DH5-alpha.GB5-alpha™ HighlightsFree of all Animal productsHigh transformation efficiency (≥1 x 108cfu/µg pUC19 DNA) Ideal for routine cloning and plasmid propagationWidely used in synthetic biology, CRISPR, and mutagenesis workflowsCompatible with NEBuilder®HiFi DNA Assembly, Gibson Assembly®, and In-Fusion®Snap AssemblyAccepts plasmids and BAC's up to ~15kbBlue-white screening via lacZΔM15 mutationReduced recombination due to recA1 mutationLownuclease activitydue to endA1 mutationFast growth and easy handling in the labKit ComponentsCompetent Cells1 x 12 mL Recovery Media1 x 10 µL Control Plasmid (pUC19 Control, 500 pg/µL) Reagents Needed for One ReactionGB5-alpha™ chemically competent cells: 50 µLDNA (orpUC19 Control, 500 pg/µL) : 2 µLRecovery medium: 1 mL