CJ236 Chemically Competent E. Coli Cells

GoldBio CJ236 ElectrocompetentE. colicells are ideally suited for use in the Kunkel method of mutagenesis. CJ236 cells have part of their Thymine replaced with deoxyuracil in the DNA due todut- andung- mutations which prevent the cells from removing uracil from DNA caused by misincorporation or dCTP deamination. CJ236 contains the F’ gene which confers Chloramphenicol resistance.Reagents Needed for One ReactionCJ236 electrocompetent cells: 25 µLDNA (or pUC19 Control, 500 pg/µL) : 0.2 µLRecovery medium: 1 mLKit ComponentsCompetent Cells1 x 12 mL Recovery Media1 x 10 µL Control Plasmid (pUC19 Control, 500 pg/µL)