BL21 Chemically Competent E. coli Cells

GoldBio’s BL21 (DE3) Chemically CompetentE. colicells are are high efficiency cells, suitable for a wide variety of applications such as transformation and routine protein expression. BL21 (DE3) chemically competent cells feature a widely used host background, a T7 expression strain, and are deficient in both Ion (1) and ompT proteases. In addition, BL21 (DE3) cells are resistant to phage T1 (fhuA2) and are B strain.BL21 (DES) chemically competent cells arefree of animal-derived productsand grown with animal-free media.BL21 (DE3) HighlightsFree of all Animal productsHigh plasmid stability and decreased plasmid transferNo DNA cytosine methylation means better restriction digestion and sequencingGalactose deficiency makes it ideal for selective systemsCarries IPTG-inducible T7 RNA polymerase for high-level expressionKit ComponentsCompetent Cells1 x 12 mL Recovery Media1 x 10 µL Control Plasmid (pUC19 Control, 500 pg/µL) Reagents Needed for One ReactionBL21 (DE3) chemically competent cells: 50 µLDNA (orpUC19 Control, 500 pg/µL) : 2 µLRecovery medium: 1 mL