BL21 (DE3) Electrocompetent E. coli Cells

GoldBio’s GB10B™ Chemically CompetentE. colicells are equivalent to DH10B cells. GB10B™cellsare high efficiency and suitable for a wide variety of applications such as cloning and sub-cloning. GB10B™ Chemically CompetentE. colicells have multiple features including the ɸ80lacZ∆M15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol. These cells also have themcrAgenotypic marker and themcrBC, mrrdeletion, which allows for cloning of DNA that contains methylcytosine and methyladenine.GB10B™ Chemically competentE. colicells are equivalent to DH10B.Reagents Needed for One ReactionGB10B™ chemically competent cells: 50 µLDNA (orpUC19 Control, 500 pg/µL) : 2 µLRecovery medium: 1 mLKit ComponentsCompetent Cells1 x 12 mL Recovery Media1 x 10 µL Control Plasmid (pUC19 Control, 500 pg/µL)