BL21 (DE3) Electrocompetent E. coli Cells

GoldBio’s BL21 (DE3) ElectrocompetentE. colicells are suitable for high efficiency transformation and routine protein expression. Increased cloning efficiencies versus typical BL21 cells makes the BL21 (DE3) Electrocompetent E. coli cells ideal for construction of complex expression libraries and feature ≥1 x 108cfu/µg efficiency with electroporation. BL21 (DE3) Electrocompetent E. coli cells have a widely used background, feature a T7 expression strain, are deficient in both lon (1) and ompT proteases. These cells are resistant to phage T1 (fhuA2) and are B strain.Reagents Needed for One ReactionBL21 (DE3) electrocompetent cells: 25 µLDNA (orpUC19 Control, 500 pg/µL) : 0.2 µLRecovery medium: 1 mLKit ComponentsCompetent Cells1 x 12 mL Recovery Media1 x 10 µL Control Plasmid (pUC19 Control, 500 pg/µL)