Bicine

Bicine is a buffering agent used in biochemistry and molecular biology that was selected and described by Goodet al.It is a zwitterionic, bis (2-hydroxyethyl) amine buffer that is useful for a pH range of 7.6 – 9.0. It is commonly used in protein crystallization, in the study of enzymatic reactions, and for electrophoresis. It is used in thin layer ion exchange chromatography for protein resolution, as well as a multiphasic buffer system for SDS-PAGE of proteins. It is soluble in water at 0°C up to 1.1 M. Bicine forms a complex with most common metals so stability constants and concentrations should be taken into consideration when choosing this buffer.Product SpecificationsBicine; N, N-bis (2-hydroxyethyl) glycine; di (hydroxyethyl) glycine; N, N- bis (hydroxyethyl) aminoacetic acidULTRA PURE GRADEFormula: C6H13NO4MW: 163.17 g/molPubChem Chemical ID:8761