D-Luciferin, Sodium Salt (Proven and Published®​)

Tested and Certified forin vivoimaging (See "Luciferin FAQ" in additional information) Luciferin is a common bioluminescent reporter used forin vivoimaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2+as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red lightin vivoat 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.Luciferin is a common reagent used throughout the biotechnology field and specifically forin vivoimaging. Luciferase labeled tumor cells, stem cells or infectious diseases are often inoculated into research animals such as rats or mice for investigation. The injection of luciferin allows for the real-time, noninvasive monitoring of disease progression and/or drug efficacy in these model systems through Bioluminescence Imaging (BLI) .Luciferin is also commonly used forin vitroresearch, including luciferase and ATP assays, gene reporter assays, high throughput sequencing and various contamination assays.Firefly Luciferin is identical to Beetle Luciferin. (See "Additional Information" for structures.) Proven and Publishedis a Registered trademarks in the U.S. Patent and Trademark Office.Applicationsin vivocellular imagingluciferase and ATP assaysgene reporter assayshigh throughput sequencingcontamination assays