Tris (Tris Base)

GoldBio Tris has the highest purity available on the market. Short for Tris (hydroxymethyl) aminomethane (THAM), Tris is an organic compound often used in buffer solutions such as TAE or TBE for electrophoresis gels. Tris is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer.A Tris buffer solution can be made by mixing Tris withTris-HCl. This prevents overshooting the pH and prevents the need to work with strong acids or bases.ApplicationsElectrophresis (TAE/TBE/TE) buffersSDS-PAGECTAB DNA extraction bufferEnzymatic assaysEllman's test