Kanamycin Monosulfate, USP Grade

GoldBio’s Kanamycin Monosulfate, USP Grade is a high-purity aminoglycoside antibiotic trusted by researchers for reliable selection in genetic engineering workflows. With its potent action against Gram-negative bacteria via 30S ribosomal subunit inhibition, this USP-grade antibiotic is widely used in bacterial cloning, inducible protein expression systems, transgenic plant development, and environmental metagenomic library screening. Its solubility in water, stability in solution, and compatibility with multi-antibiotic systems make it a foundational tool in molecular biology, synthetic biology, and functional genomics. Choose GoldBio kanamycin for precise, reproducible selection in your research pipeline.Kanamycin is an aminoglycoside antibiotic isolated fromStreptomyces kanamyceticus. It is effective against gram-positive and gram-negative bacteria as well asMycoplasmaspecies. Kanamycin is commonly used to select for bacteria that have been transformed with a kanamycin-resistant gene and plant tissues that have theNPT II (APH3) gene incorporated in them. Kanamycin is very soluble in aqueous solution and is typically used in concentrations of 50-100 µg/ml.Aminoglycoside antibiotics are composed of amino groups attached to glycosides. They bind the 30s ribosomal subunit, causing misreading of the mRNA sequence and inhibition of translocation. Consequently, protein synthesis is inhibited.Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic (s) to prescribe for various infections.TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS AT GOLD BIOTECHNOLOGY LAB.Request a Bulk QuoteNeed larger quantities for your research or production runs? Usethis form to request a custom bulk quoteand secure the best value for your lab. Your account manager will follow up with you promptly to provide tailored pricing and support for your order.Common Research Applications for Kanamycin Monosulfate (Click each for more information) Selection of Bacteria with Kanamycin Resistance PlasmidsPurpose: Enables selective growth of genetically modified bacteria harboring kanamycin resistance genes (e.g., aph (3') -IIa) .Mechanism: Kanamycin inhibits bacterial protein synthesis by binding to the 30S ribosomal subunit, halting translation; only resistant transformants expressing kanamycin-modifying enzymes survive.Applications: Essential for plasmid propagation, gene cloning, and recombinant protein production inE. coliand other Gram-negative hosts.Messing, J., & Vieira, J. (1991) . New pUC-derived cloning vectors with different selectable markers and DNA replication origins.Gene, 100 (1), 189–194.Plant Genetic TransformationPurpose: Selects for transformed plant cells carrying the nptII gene conferring kanamycin resistance following genetic transformation.Mechanism: InAgrobacterium-mediated or biolistic transformation, kanamycin eliminates non-transformed cells, allowing only transgenic cells to proliferate.Applications: Widely used in generating transgenic crops, especially in dicotyledonous and model plants such asArabidopsisand tobacco.Hiei, Y., Ohta, S., Komari, T., & Kumashiro, T. (1994) . Efficient transformation of rice (Oryza sativaL.) mediated byAgrobacteriumand sequence analysis of the boundaries of the T-DNA.Plant Journal, 6 (2), 271–282.Protein Expression in Inducible SystemsPurpose: Selects for bacteria carrying expression plasmids encoding inducible systems (e.g., T7, araBAD) with kanamycin resistance.Mechanism: Ensures propagation of only properly transformed bacteria prior to induction of recombinant protein production.Applications: Used in BL21 (DE3) and similar strains for production of high-yield proteins for structural biology or therapeutic research.Studier, F. W., & Moffatt, B. A. (1986) . Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.Journal of Molecular Biology, 189 (1), 113–130.Synthetic Biology for Circuit Design and ModularityPurpose: Supports modular genetic engineering through distinct selection markers across multiple plasmids.Mechanism: Kanamycin resistance enables stable maintenance of one or more plasmids in engineeredE. coli, allowing combinatorial gene assembly.Applications: Used in synthetic circuits, CRISPR assemblies, and metabolic pathway engineering.Mutalik, V. K., et al. (2013) . Precise and reliable gene expression via standard transcription and translation initiation elements.Nature Methods, 10 (4), 354–360.Metagenomic Library Screening and Functional Gene MiningPurpose: Facilitates construction and maintenance of metagenomic libraries in kanamycin-resistant vectors for function-based discovery.Mechanism: Environmental DNA is cloned into large-insert vectors (fosmids/cosmids) carrying kanamycin resistance, then introduced intoE. colifor screening novel enzymes or biosynthetic genes.Applications: Enables mining of uncultured microbial genomes for antibiotics, biofuels, or novel metabolic capabilities.Rondon, M. R., et al. (2000) . Cloning the soil metagenome: a strategy for accessing the genetic and functional diversity of uncultured microorganisms.Applied and Environmental Microbiology, 66 (6), 2541–2547.BenefitsHigh-Purity Antibiotic: USP grade ensures reliability and consistency in demanding research applications.Effective Selection Agent: Delivers robust elimination of non-transformed cells in both microbial and plant systems.Cross-System Versatility: Compatible with bacterial cloning, plant transformation, synthetic biology, and metagenomics.Stable and Water-Soluble: Enables convenient stock solution preparation with strong storage performance.Supports Multimodal Engineering: Ideal for workflows requiring multiple antibiotic resistances across complex plasmid systems.