G418 Sulfate

G-418 sulfate, also known as geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is commonly used in plant and mammalian cell culture as a selectable marker. It can be used to select for cells transformed with an iNOS promoter construct or neomycin resistance gene.G-418 functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation. Resistance to G-418 is conferred through thenptllgene in plant cells and the neo gene in mammalian cells, which encode aminoglycoside 3'-phosphotransferase. For gene selection applications, a kill curve can be made to determine the minimum effective G418 concentration to kill non-resistant cells. G418 disulfate is freely soluble in water.This product has been tested against both sensitive and resistant cells at Gold Biotechnology Labs.Product Specifications:G418; GeneticinFormula: C20H40N4O10· 2H2SO4MW: 692.71 g/mol