5-Fluoroorotic acid monohydrate (5-FOA)

5-Fluoroorotic Acid (5-FOA) is widely used in the field of yeast molecular genetics. The toxicity of 5-FOA to yeast cells with a functioning URA3 gene which encodes orotidine-5′-P decarboxylase (OMP decarboxylase), makes it especially useful for the selection and identification of mutant yeast strains. 5-FOA is most often applied withSaccharomyces cerevisiae (URA3), although it has been used in other strains includingSchizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3) and the bacteriumE. coli (pyrF) . The use of 5-FOA in the yeast 2-hybrid system allows for the construction of activation domain hybrid libraries to identify protein-protein interactions.Product Specifications:5-Fluoroorotic acid monohydrate (5-FOA) 5-Fluorouracil-4-carboxylic acidMOLECULAR BIOLOGY GRADEFormula: C5H3FN2O4• H2OMW: 192.10 g/molWhite to slightly off-white crystalline powder