Protein A Magnetic Agarose Beads

Protein A Magnetic Agarose Beads are designed to provide the easiest and simplest method of monoclonal and polyclonal antibody purification. The antibodies are retained to the magnetic resin which are also covalently conjugated with recombinant Protein A. Magnetic agarose beads provide a convenient and quick method for purification without the need for pipetting or centrifugation. Washing, binding and elution steps will require a magnetic device.Protein A is a cell wall component ofStaphylococcus aureus. It consists of a single polypeptide chain shaped as a cylinder, which contains five antibody–binding domains. These high affinity regions are specifically bonded to Fc region of the immunoglobulins (specially IgGs of different species) . Protein A is temperature stable and it retains its native conformation even in the presence of denaturing agents.Protein A Magnetic Beads are supplied as a 50% suspension of magnetic beadsin 20% ethanol (Each 1000 μl of suspension contains 500 μl of magnetic beads.) Binding Capacity: ~10 mg human IgG / ml resin.