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Highest Density Nickel

GoldBio’s new Highest Density Nickel Agarose is a high capacity resin manufactured with a polychelator ligand. The product was developed to work in the presence of EDTA and DTT without any loss of performance. Its specificity and stability allows a one-step purification, eliminating the need for pretreatment of samples that cause nickel stripping.Our Highest Density Nickel Agarose is supplied as a suspension in 50% aqueous suspension containing 20% ethanol. Purifications can be performed using 20mM EDTA and 20mM DTT and can work in both native as well as denaturing conditions.Product SpecificationsBead Geometry/Size: Spherical - ~50-150 µmCrosslinking: Highly CrosslinkedAgarose: 6%Chemical Stability:20mM DTT20mM EDTA8M urea6M guanidine hydrochloride30% acetonitrile100% methanol100% ethanolBuffer solutions at pH 4 - 9Loading Capacity: >60 μmol me2+/ml gelStatic Binding Capacity: ≥80 mg/ml gelAntimicrobial Agent: 20% ethanol

Product Specifications

Product Datasheet

https://www.goldbio.com/documents/1013/Affinity His-Tag Purification Troubleshooting.pdf; https://www.goldbio.com/documents/1014/Affinity vs Specificity - His Tag.pdf; https://www.goldbio.com/documents/1047/Affinity His-Tag Purification Utilizing Chelating Agarose Beads.pdf; https://www.goldbio.com/documents/1050/GoldBio His-Tag Column Prep Protocol.pdf; https://www.goldbio.com/documents/1473/Affinity His-Tag Purification utilizing Chelating Agarose Bead Spin Columns Protocol.pdf
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